Day 2 in BPT lab

Yo.. It’s our 2nd day of lab and our lab is at 10 am! Wow so early!! Hahaha….
But our lab was very short…
So we can go home continue our sleep…

In the lab, Ms Ang showed us our LB/Amp/Ara plate where we had culture the pGLO transformed E.coli… I was excited to see how is our result..

BUT..!!

When we observed our streak plate under the UV light, there wasn’t any growth at all! Likewise goes to the other group. There isn’t any growth is maybe due to some problems with our e.coli or maybe our streaking technique got problem.
So, we have to use the “back-up” one which was done by our lab tech (Yong Hao).

Firstly, we transferred several colonies of pGLO transformed E.coli from a fresh LB/Amp/Ara plate to the shaker flask containing 100ml LB medium with ampicillin. Aseptic technique such as working near the Bunsen flame, burn the cap of the shaker flask etc was carried out…

After transferring, the flask was place in shaking incubator and incubated at 32oC for the rest of the day.

Since my group mates have explained the pGLO transformed E.coli, I’m now going to explain the ingredient of LB/Amp/Ara plate

LB ( Luria-Bertani): LB is used for the growth of E.coli by providing nutrients to them.

Ampicillin (Amp) : Ampicillin is a selective agent that used to confirm that the bacteria have uptake the genes. pGLO transformed E.coli plasmid consist of an antibiotic resistant genes and if the bacteria have successfully take up the needed genes, the bacteria will become ampicillin resistance. Thus the E.coli are able to grown on the LB/Amp/Ara plate which consist of ampicillin.

Arabinose (Ara): This sugar helps to turn on pGLOgenes.