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In this blog, we're blogging on the whole practical we have done in reeeeal life and blogging on what we have learnt in the practical! This practical is mainly on the bioreactor we have used and how we use the bioreactor to grow our culture! Yeap.
The overall objective of this practical is to allow us to successfully cultivate the pGLO transformed E.coli in the bioreactor and for us to learn and know more on bioreactors.
Some extra info of the objectives of the the individual expt conducted during lab..
Expt 1:
-To familiarize students with the parts and components of microbial and mammalian bioreactors.
-To introduce the basic operation procedure of a bioreactor
Expt 2:
-To describe the steps to prepare a bioreactor
-To prepare the media for seed culture and scale up fermentation
-To prepare seed culture for scale up fermentation
Expt 3:
-To carry out scale up fermentation process to increase the yield of desired protein product
-To monitor cell growth and product formation through manual sampling and computer data logging
Expt 4:
-To isolate and purify the final product
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and finally,
Hope YOU guys have an enjoyable learning journey here!
Yummy,
Buffalo Wings..
